Lineage specific FMDV Real-time RT-PCR assays

Supplied here are a series of leaflets desibribing real-time RT-PCR assays that have been designed to detect different foot-and-mouth disease virus lineages in different parts of the world.

Each leaflet describes an assay for particular FMDV lineages expected to be circulating in a geographical location. A summary of all these real-time RT-PCR assays (found in all the leaflets) can be found here.

East Africa

This set of specific assays was designed for the detection of FMDV strains currently circulating in the region of East Africa especially Tanzania, Uganda and Kenya (Bachanek-Bankowska et al., 2016)

Designed to detect: O/EA-2, O/EA-4, A/Africa/G-I, SAT 1/I(NWZ) and SAT 2/IV

EastAfrica

West Eurasia

This set of real-time RT-PCR type-specific as-says was designed for the detection of FMDV strains currently circulating in West Eurasia (Reid et al., 2014).

Designed to detect: O/ME-SA/PanAsia-2, A/ASIA/Iran- 05, Asia1/ASIA/Group 1, Asia1/ASIA/Group 2 and Asia1/ASIA/Group 6

West Euroasia

Ind-2001

This FMDV-O Ind-2001-specific real-time RT-PCR is a molecular tool for detection of foot-and-mouth Disease virus O/ME-SA/Ind2001 lineage (Knowles et al., 2014).

This lineage was originally detected on the Indian sub-continent, but has since spread westwards through the Middle East to North Africa and eastwards into Southeast Asia

Designed to detect: O/ME-SA/Ind-2001d

Ind-2001

SAT 2 - Egypt

This real-time RT-PCR is a molecular tool for detection of Foot-and-mouth disease virus SAT 2/VII topotype and was developed in response to the SAT 2 outbreak in Egypt in 2012 (Ahmed et al., 2012).

Outside of Egypt this lineage has been detected in Libya, Palestinian Autonomous Territories, Eritrea and Cameroon.

Designed to detect: SAT 2/VII

SAT2 Egypt

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